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Sangon Biotech spin column animal total rna purification kit
Spin Column Animal Total Rna Purification Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spin+column+animal+total+rna+purification+kit/column+dna+extraction+gel+kit+sanprep/pm42297343-57-6-34
Average 86 stars, based on 1 article reviews
spin column animal total rna purification kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Purification:

Article Title: Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway.
Article Snippet: .. Total RNA was extracted from livers using a spin column animal total RNA purification kit (Sangon Biotech, Shanghai, China) and was reverse-transcribed using M-MuLV first-strand cDNA synthesis kit (Sangon Biotech, Shanghai, China) https://doi.org/10.1371/journal.pone.0333872.g001 according to the manufacturer’s protocol. .. Real-time quantitative PCR (qPCR) was performed on a LightCycler480 system (Roche, Switzerland) using TB Green premix Ex Taq II mix (TaKaRa, Dalian, China).

Article Title: Arsenic sulfide enhances the therapeutic effect of hepatocellular carcinoma immunotherapy through STAT3-THBS1/CD47 pathway
Article Snippet: Immunoblot signals were acquired with the Amersham Imager 600 (General Electric Company, Boston, MA, USA). .. RNA was isolated with Spin Column Animal Total RNA Purification Kit (Sangon Biotech) and reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was amplified using SYBR-Green PCR Master Mix (Thermo Fisher Scientific). ..

Article Title: Effects of curcumin supplementation in high-fat diet on growth performance, antioxidant capacity and lipid metabolism of spotted sea bass (Lateolabrax maculatus).
Article Snippet: This study aimed to investigate the effects of curcumin supplementation in high-fat diets on growth performance, antioxidant capacity and lipid metabolism in spotted sea bass (Lateolabrax maculatus).. Six experimental diets were formulated: a normal-fat diet (NF), a high-fat diet (HF), and four experimental diets derived from the HF supplemented with 0.02%, 0.04%, 0.08%, and 0.16% curcumin.. A total of 450 healthy spotted sea bass, with an initial body weight of (2.00 ± 0.16) g, were selected and randomly allocated into 6 groups (3 replicates per group, 25 fish per replicate).

Article Title: Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway
Article Snippet: .. Total RNA was extracted from livers using a spin column animal total RNA purification kit (Sangon Biotech, Shanghai, China) and was reverse-transcribed using M-MuLV first-strand cDNA synthesis kit (Sangon Biotech, Shanghai, China) according to the manufacturer’s protocol. .. Real-time quantitative PCR (qPCR) was performed on a LightCycler480 system (Roche, Switzerland) using TB Green premix Ex Taq II mix (TaKaRa, Dalian, China).

Article Title: A new utilization of saponins from the root of Clematis chinensis Osbeck: Hypouricemia effect.
Article Snippet: The exploration of resource plants is conducive to expanding the scope of their rational utilization and application.. Clematis chinensis Osbeck, known as Wei-Ling-Xian (WLX) in China, is a resource plant that has diverse applications and developments.. However, the utilization and development of WLX resources remain insufficient.

Article Title: Schisandrol A induces cardiac developmental toxicity in zebrafish (Danio rerio) via disruption of calcium homeostasis, oxidative stress, and apoptosis.
Article Snippet: Schisandrol A (SA) is a major lignan component of Schisandra chinensis with anti-inflammatory and antioxidant activities, yet its developmental and cardiac safety profile remains unclear.. In this study, a zebrafish (Danio rerio) embryo model was used to evaluate the toxicity of SA and its underlying mechanisms following exposure to different concentrations of SA (ranging from 0 to 160 μg/mL, with safe concentrations of 10, 20, and 40 μg/mL for mechanistic studies from 6 to 96 h post-fertilization (hpf).. SA induced a dose-dependent increase in mortality, with an LC50 of 62.5 μg/mL at 96 hpf.

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling
Article Snippet: Cell/Tissue Total RNA Isolation Kit , Vazyme Biotech , Cat# RC112. .. Spin Column Animal Total RNA Purification Kit , Sangon Biotech , Cat# B518651. .. SYBR qPCR Master Mix , Vazyme Biotech , Cat# Q712.

Reverse Transcription:

Article Title: Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway.
Article Snippet: .. Total RNA was extracted from livers using a spin column animal total RNA purification kit (Sangon Biotech, Shanghai, China) and was reverse-transcribed using M-MuLV first-strand cDNA synthesis kit (Sangon Biotech, Shanghai, China) https://doi.org/10.1371/journal.pone.0333872.g001 according to the manufacturer’s protocol. .. Real-time quantitative PCR (qPCR) was performed on a LightCycler480 system (Roche, Switzerland) using TB Green premix Ex Taq II mix (TaKaRa, Dalian, China).

Article Title: Arsenic sulfide enhances the therapeutic effect of hepatocellular carcinoma immunotherapy through STAT3-THBS1/CD47 pathway
Article Snippet: Immunoblot signals were acquired with the Amersham Imager 600 (General Electric Company, Boston, MA, USA). .. RNA was isolated with Spin Column Animal Total RNA Purification Kit (Sangon Biotech) and reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was amplified using SYBR-Green PCR Master Mix (Thermo Fisher Scientific). ..

Article Title: Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway
Article Snippet: .. Total RNA was extracted from livers using a spin column animal total RNA purification kit (Sangon Biotech, Shanghai, China) and was reverse-transcribed using M-MuLV first-strand cDNA synthesis kit (Sangon Biotech, Shanghai, China) according to the manufacturer’s protocol. .. Real-time quantitative PCR (qPCR) was performed on a LightCycler480 system (Roche, Switzerland) using TB Green premix Ex Taq II mix (TaKaRa, Dalian, China).

cDNA Synthesis:

Article Title: Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway.
Article Snippet: .. Total RNA was extracted from livers using a spin column animal total RNA purification kit (Sangon Biotech, Shanghai, China) and was reverse-transcribed using M-MuLV first-strand cDNA synthesis kit (Sangon Biotech, Shanghai, China) https://doi.org/10.1371/journal.pone.0333872.g001 according to the manufacturer’s protocol. .. Real-time quantitative PCR (qPCR) was performed on a LightCycler480 system (Roche, Switzerland) using TB Green premix Ex Taq II mix (TaKaRa, Dalian, China).

Article Title: Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway
Article Snippet: .. Total RNA was extracted from livers using a spin column animal total RNA purification kit (Sangon Biotech, Shanghai, China) and was reverse-transcribed using M-MuLV first-strand cDNA synthesis kit (Sangon Biotech, Shanghai, China) according to the manufacturer’s protocol. .. Real-time quantitative PCR (qPCR) was performed on a LightCycler480 system (Roche, Switzerland) using TB Green premix Ex Taq II mix (TaKaRa, Dalian, China).

Article Title: Schisandrol A induces cardiac developmental toxicity in zebrafish (Danio rerio) via disruption of calcium homeostasis, oxidative stress, and apoptosis.
Article Snippet: Schisandrol A (SA) is a major lignan component of Schisandra chinensis with anti-inflammatory and antioxidant activities, yet its developmental and cardiac safety profile remains unclear.. In this study, a zebrafish (Danio rerio) embryo model was used to evaluate the toxicity of SA and its underlying mechanisms following exposure to different concentrations of SA (ranging from 0 to 160 μg/mL, with safe concentrations of 10, 20, and 40 μg/mL for mechanistic studies from 6 to 96 h post-fertilization (hpf).. SA induced a dose-dependent increase in mortality, with an LC50 of 62.5 μg/mL at 96 hpf.

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

Isolation:

Article Title: Arsenic sulfide enhances the therapeutic effect of hepatocellular carcinoma immunotherapy through STAT3-THBS1/CD47 pathway
Article Snippet: Immunoblot signals were acquired with the Amersham Imager 600 (General Electric Company, Boston, MA, USA). .. RNA was isolated with Spin Column Animal Total RNA Purification Kit (Sangon Biotech) and reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was amplified using SYBR-Green PCR Master Mix (Thermo Fisher Scientific). ..

Amplification:

Article Title: Arsenic sulfide enhances the therapeutic effect of hepatocellular carcinoma immunotherapy through STAT3-THBS1/CD47 pathway
Article Snippet: Immunoblot signals were acquired with the Amersham Imager 600 (General Electric Company, Boston, MA, USA). .. RNA was isolated with Spin Column Animal Total RNA Purification Kit (Sangon Biotech) and reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was amplified using SYBR-Green PCR Master Mix (Thermo Fisher Scientific). ..

SYBR Green Assay:

Article Title: Arsenic sulfide enhances the therapeutic effect of hepatocellular carcinoma immunotherapy through STAT3-THBS1/CD47 pathway
Article Snippet: Immunoblot signals were acquired with the Amersham Imager 600 (General Electric Company, Boston, MA, USA). .. RNA was isolated with Spin Column Animal Total RNA Purification Kit (Sangon Biotech) and reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was amplified using SYBR-Green PCR Master Mix (Thermo Fisher Scientific). ..

Polymerase Chain Reaction:

Article Title: Arsenic sulfide enhances the therapeutic effect of hepatocellular carcinoma immunotherapy through STAT3-THBS1/CD47 pathway
Article Snippet: Immunoblot signals were acquired with the Amersham Imager 600 (General Electric Company, Boston, MA, USA). .. RNA was isolated with Spin Column Animal Total RNA Purification Kit (Sangon Biotech) and reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was amplified using SYBR-Green PCR Master Mix (Thermo Fisher Scientific). ..

Synthesized:

Article Title: Schisandrol A induces cardiac developmental toxicity in zebrafish (Danio rerio) via disruption of calcium homeostasis, oxidative stress, and apoptosis.
Article Snippet: Schisandrol A (SA) is a major lignan component of Schisandra chinensis with anti-inflammatory and antioxidant activities, yet its developmental and cardiac safety profile remains unclear.. In this study, a zebrafish (Danio rerio) embryo model was used to evaluate the toxicity of SA and its underlying mechanisms following exposure to different concentrations of SA (ranging from 0 to 160 μg/mL, with safe concentrations of 10, 20, and 40 μg/mL for mechanistic studies from 6 to 96 h post-fertilization (hpf).. SA induced a dose-dependent increase in mortality, with an LC50 of 62.5 μg/mL at 96 hpf.

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.



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